CSIR NET Phd antrance Exam Solved Model Question Paper
#1
Can be had for additional preparation for CSIR NET / DBT-JRF / ICMR- JRF/ JNU Phd entrance exam model question paper with answers----Download from the link attached...

Courtesy: Bio Frontiers
Prepared By: Suborno Joti, Indian Institute of Chemical Biology (IICB), Kolkata, India &
Kartik Guchhait, Department of Physiology, Vidyasagar University, Midnapore, India


1. During transcription process sigma subunit recognizes promoter sites of DNA and helps RNA polymerase enzyme to efficiently binding with the DNA. Which of the following statements is true regarding the prokaryotic transcription
(a)    In E.coli bacterial promoter, there is two consensus sequence present. One is the - 10 region and another is +35 region from the transcription start site.
(b)    Sigma -28 binds with -10 consensus sequence of the bacterial promoter and helps in oxidative stress response signalling.
©Â Â   AConsensus sequence defines different bases or amino acids in different related protein or nucleic acid sequences.
(d)    The alpha subunit of RNA polymerase recognizes and interacts with the UP elements of bacterial system.

2.During transcription primer is not needed to synthesize RNA strand. The synthesis of RNA can start dc-novo. Which of the following options is correct about prokaryotic transcription -
(a)    In prokaryotic promoter. -10 region is called the pribnow box, which in eukaryotes is called GC box.
(b)    The core enzyme of RNA polymerase in prokaryotic microorganism has the ability to initiate transcription.
©Â Â   RNA polymerase enzyme remains in static phase during initial transcription and drag the downstream DNA towards itself.
(d)    The first nucleotide present in the newly synthesized transcript is always pppC or pppT.

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